Asked 7/7/2008
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how dry heat kills microbes in 300 C and autoclaves at 135 C. please clarify.? if microbes are killed at 135 C in an autoclave for 5 minutes sterilizing time, why the dry heat process need 300 C for 3 hours to kill the same microbes. |
Answer 1/1 - Submitted 7/7/2008
During autoclaving microbes, particularly those containing heat resistant endospores are killed by heat shock as steam condenses and breaks open the cells.
The same heat resistant endospores require much higher temperatures to kill them using dry heat.
Hope this helps!
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